Extraction and purification of C-phycocyanin from dry Spirulina powder and evaluating its antioxidant, anticoagulation and prevention of DNA damage activity.

C-Phycocyanin (C-PC) is a phycobiliprotein found in blue green algae, such as Spirulina platensis, is often used as a dietary nutritional supplement and exhibit a variety of pharmacological properties. In this regard, extraction, partial purification and antioxidant, anticoagulation and prevention of DNA damage activity of C-PC was investigated. In the present study, a simple and efficient method to extract C-PC from Spirulina platensis dry powder is reported. The extractions were carried out using two different methods: cold maceration and sonication method. The extraction using cold maceration method proved to be the most efficient method. Obtained crud CPC was purified by ammonium sulphate precipitation, dialysis and gel filtration and presented a final extraction yield of 3.27±0.09 mg/ml with a purity ratio of 2.317±0.08. When it was evaluated as an antioxidant in vitro, it was able to scavenge nitric oxide. C-PC showed significant anticoagulation and prevention of DNA damage activity.

Effect of nitrogen sources on the production of invertase by yeast saccharomyces cerevisiae 3090.

Invertase from Saccharomyces cerevisiae is high cost enzyme and primarily used in the confectionary industry. For large scale production of the enzyme, feasible synthetic medium with appropriate supplemented nutrients are required. The effect of carbon source on invertase production is well known, but little is known about the effect of different nitrogen source. The aim of the present study is to see the effect of different nitrogen sources on the production of invertase in submerged fermentation by Saccharomyces cerevisiae. It was found that when urea as nitrogen source is added in a little amount to the fermentation medium it showed a marked increase in Invertase production.

Antimicrobial Activity of Extracellularly Synthesized Silver Nanoparticles using Lactobacillus Species Obtained from VIZYLAC Capsule.

The development of green experimental processes for the synthesis of nanoparticles is a need in the field of nanotechnology. In present study deals with the extracellular biosynthesis of silver nanoparticles using VIZYLAC capsule containing about 106 Lactobacillus. Two different Species of Lactobacillus obtained from VIZYLAC capsule were used for the synthesis of silver nanoparticles. Silver nanoparticles were formed by treatment of aqueous solution of AgNO3 (1mM) with supernatant obtained from 24 hour grown culture of Lactobacillus Spp. The synthesis of silver nanoparticles was achieved within 3 days of incubation. UV-visible specrophotometric analysis was carried out to assess the formation of silver nanoparticles. Silver nanoparticles were further characterized using Nanoparticle Tracking Analyzer (NTA), Transmission Electron Microscopy (TEM) and Energy Dispersisve X-ray Spectrum (EDX). Synthesized silver nanoparticles showed antibacterial activity against hospital isolate of Proteus mirabilis (Multiple Drug Resistent), Salmonella typhi, and Klebsiella pneumoniae. The antifungal activity was checked against Candida albicans (hospital isolate) and Aspergillus niger (NCIM 616). The extracellular synthesis of size and shape controlled, monodispersed silver nanoparticles using Lactobacillus species appears to be cost effective and eco-friendly.